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KMID : 0545120100200060988
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 6 p.988 ~ p.994
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The Melanin Synthesis inhibition and Radical Scavenging Activities of Compounds Isolated from the Aerial Part of Lespedeza cyrtobotrya
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Lee Mi-Yeon
Kim Jin-Hee
Choi Jung-Nam
Kim Ji-Young
Hwang Geum-Sook
Lee Choong-Hwan
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Abstract
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The EtOAc fraction of Lespedeza cyrtobotrya showed mushroom tyrosinase inhibitory and radical scavenging activity. Four active compounds were isolated based on LH-20 chromatography and HPLC, and the structures were elucidated on the basis of their LC-MS and NMR spectral data, as 2-(2,4-Dihydroxyphenyl)-6-hydroxybenzofuran (1), eriodictyol-7-O-glucopyranoside (2), haginin A (3), and dalbergioidin (4), respectively. 2-(2,4-Dihydroxyphenyl)-6-hydroxybenzofuran (1) showed mushroom tyrosinase inhibitory activity with an IC50 value of 5.2 μM and acted as a competitive inhibitor. Furthermore, 37.3 μM of compound 1 reduced 50% of the melanin content on a human melanoma (MNT-1) cells. The radical scavenging activity of 2-(2,4-dihydroxyphenyl)-6-hydroxybenzofuran (1), eriodictyol-7-O-glucopyranoside (2), haginin A (3), and dalbergioidin (4) was shown with IC50 values of 11.0, 24.5, 9.0 and 36.5 μM in an ABTS system and with IC50 values of 42.7, 36.0, 37.7 and 61.7 μM in a DPPH system, respectively. The mushroom tyrosinase inhibitory activity of EtOAc fraction of Lespedeza cyrtobotrya was contributed by compound 1, 3 and 4, and radical scavenging activity of it was contributed by compound 1-4.
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KEYWORD
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Tyrosinase, Human melanoma (MNT-1) cell, Radical scavenging, Lespedeza cyrtobotrya
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